Coming soon: Plasmid for generating single marker Trypanosoma brucei cell lines expressing the T7 RNA polymerase and the tetracyline repressor:

This plasmid was developed to enable rapid, reproducible and efficient introduction of the T7 RNA polymerase and tetracycline repressor protein into any strain of Trypanosoma brucei.
The is the plasmid used to generate the SMoX (Single Marker Oxford) series of cell lines in TREU927 and 427 strains.
the plasmid is highly-modular in design and both the T7 RNA polymerase and tetracycline repressor contain N-terminal nuclear localisation sequences.
Poon et al. (2012) OpenBiology

Plasmid	map	GenBank	GCK	Marker	Transgene
pSMOx				PUR	T7RNAP, TETr

Plasmids for inducible transgene expression:

Minichromosomal repeat-integrating constructs for inducible expression of transgenes. Vectors are highly-modular expression vectors described in Kelly et al. (2007) MBP 154:103-9

Plasmid	map	GenBank	GCK	Constitutive promoter	Marker	Inducible promoter	Transgene
pDex577-G				rDNA	BLE	T7	TY-GFP-TY
pDex577-Y				rDNA	BLE	T7	TY-YFP-TY
pDex577-C				rDNA	BLE	T7	TY-GFP-TY

Plasmids for endogenous locus tagging:

This set of plasmids are highly modular constructs for easy tagging of endogenous genes at either N- or C-termini. They are described in Kelly et al. (2007) MBP 154:103-9

Plasmid	map	GenBank	GCK	Marker	Tag
pEnT5-G				HYG	TY-GFP-TY
pEnT5-Y				HYG	TY-YFP-TY
pEnT5-C				HYG	TY-CFP-TY
pEnT6B-G				BLAST	TY-GFP-TY
pEnT6B-Y				BLAST	TY-YFP-TY
pEnT6B-C				BLAST	TY-CFP-TY
pEnT6P-G				PURO	TY-GFP-TY
pEnT6P-Y				PURO	TY-YFP-TY
pEnT6P-C				PURO	TY-CFP-TY